Female gliders reach sexual maturity late in the first year of life; males early in their second year.

In captivity, gliders might produce between three and four litters per year as compared to once a year in the wild.

Mating usually occurs late at night and typically results in one or two offspring, although litters of three or four are possible.

Like most marsupials, the female has a pouch where the young gliders nurse, develop, and are protected. Newborn gliders, called joeys, make their way into the pouch after birth and remain there for approximately 10 weeks. Once they emerge from the pocket, the male will help take with their care.

After 16-17 weeks, the young are fully weaned and ready to be independent.